Phosphatidylserine-mediated phagocytosis involves unique cytoskeletal dynamics and coreceptors TREM2, CD14 and integrin α_Mβ₂

D Vorselen*, R Kamber*, RLD Labitigan, AP van Loon, MK Delgado, MC Bassik, JA Theriot

In Review. on Biorxiv

Dynamics of phosphatidylserine-mediated phagocytosis.

D Vorselen

Biochemical Society Transactions 50: 1281(2022)

DOI

F-actin organization and target constriction during primary macrophage phagocytosis is balanced by competing activity of myosin-I and myosin-II.

SR Barger*, D Vorselen*^,† , NC Gauthier, JA Theriot, M Krendel^†

MBOC (2022)

DOI

Phagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis.

D Vorselen*, SR Barger*, Y Wang, W Cai, JA Theriot, NC Gauthier, M Krendel

eLife 10, e68627 (2021)

DOI

Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell-target interactions.

D Vorselen, Y Wang, MM de Jesus, PK Shah, M Footer, M Huse, W Cai, JA Theriot

Nature Communications 11, 20 (2020)

DOI

This paper introduced a technique that uses deformable microparticles as cellular force sensors for studying immune cell dynamics. The technology by now has been shared with >40 research groups all over the world!

A mechanical perspective on phagocytic cup formation.

D Vorselen*, RLD Labitigan*, JA Theriot

Current Opinion in Cell Biology 66, 112-122 (2020)

DOI

Systematic Identification of General and Context-Specific Regulators of Phagocytosis Using Magnetic Genome-Wide CRISPR Screens.

MS Haney, CJ Bohlen, DW Morgens, JA Ousey, AA Barkal, CK Tsui, BK Ego, R Levin, RA Kamber, H Collins, A Tucker, A Li, D Vorselen, RLD Labitigan, E Crane, E Boyle, L Jiang, J Chan, E Rincón, WJ Greenleaf, B Li, MP Snyder, IL Weissman, JA Theriot , SR Collins, BA Barres, MC Bassik

Nature Genetics 50, 1716 (2018)

DOI

The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis.

D Vorselen, SM van Dommelen, R Sorkin, MC Piontek, J Schiller, ST Döpp, SAA Kooijmans, BA van Oirschot, BA Versluijs, MB Bierings, R van Wijk, RM Schiffelers, GJL Wuite, WH Roos

Nature Communications 9, 4960 (2018)

DOI

Competition between Bending and Internal Pressure Governs the Mechanics of Fluid Nanovesicles.  

D Vorselen, FC MacKintosh, WH Roos, GJL Wuite

ACS Nano 11, 2628-2636 (2017)

DOI

Generic Indicators for Loss of Resilience Before a Tipping Point Leading to Population Collapse. 

L Dai*, D Vorselen*, KS Korolev and J Gore

Science 336, 1175 – 1177 (2012)

DOI